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Welcome to OP Singh's Lab Page
Molecular Biology Laboratory
National Institute of Malaria Research, Delhi, India
Lab Alumni
- Surendra K Prajapati, PhD (2011-14), currently in Uniformed Services University of the Health Sciences, Bethesda
- Prerna Bali, PhD (2013-2014), currently Dr DS Kothari Fellow in Jawahar Lal University, Delhi
- Rajababu S Kushwah, PhD (2011-2015), postdoctoral fellow (visiting scholar) in University of California, San Diego
- Divya Sharma, PhD (Nov 2012-March 2016)
- Manila Lather, PhD (Nov 2012-March 2016), currently teaching in Goverment College at Bahadurgarh
- Cherry L Dykes, PhD (Sep 2009-July 2016)
April 2017
New postdoctoral fellow joined lab
Dr Deepa Jha joined our lab as a ICMR Centenary Postdoctoral Fellow..
Deepa has recently completed her PhD degree from Delhi University (South Campus).
Her current research assignment is to study the role of some detoxifying enzymes in insecticide resistance in malaria vectors
9 May 2017
Farewell to Dr Rajababu Kushwah
Dr Rajababu Kushwah is leaving to University of California San Diego as Visiting Scholar for two years immediately after joining Tata Institute for Genetics and Society, Bengaluru, as a Scientist.
Dr Kushwah worked in my lab as ICMR-Senior Research Fellow for 4 years on the knockdown resistance (kdr) in Aedes aegypti and recently completed his PhD degree from IGNOU University
We wish every success in his life
9 May 2017
Farewell to Dr Rajababu Kushwah
Dr Rajababu Kushwah is leaving to University of California San Diego as Visiting Scholar for two years immediately after joining Tata Institute for Genetics and Society, Bengaluru, as a Scientist.
Dr Kushwah worked in my lab as ICMR-Senior Research Fellow for 4 years on the knockdown resistance (kdr) in Aedes aegypti and recently completed his PhD degree from IGNOU University
We wish every success in his life
May 2017
DST (WOS-B) fellowship awarded to Ankita
Ms Ankita Sindhania bagged a three-year felowship from DST under Women Scientist Scheme-B (WOS-B) to work on vectorial competence of a newly identified malaria vector.
Ankita is working in our lab since 2015, when she joined as DST's-Women Intern
May 2017
New project funding sanctioned by DST-SERB
DST-SERB has principally approved award of a research project on mechanisms of pyrethroid resistance in Anopheles stephensi and An. culicifacies (Budget: 86 lakhs).
30 June 2017
Farewell to Nabmita and Shri Bhagwan
Today we gave farewell to two of our lab member Dr Nabmita and Shri Bhagwan. Dr Nabmita was a postdoctoral fellow aand her project tenure ended today. Mr. Shri Bagwan was a regular technician in my lab and superannuated today after completing his service tenure successfully.
We will miss you Dr Nabmita and Shri Bhagwan!
1 July 2017
Congratulations to Dr Prashant Mallick for accepting regular Faculty Position in NIMR
Dr Prashant Mallick has been offered regular position of 'Scientist-C' in NIMR. He has been working in our lab since last 7 years as SRF and Scientist B in a research project .
He will continue to work in our lab
21 July 2017
Congratulations to Cherry for graduating with PhD degree!
Cherry L Dykes, my first PhD student, graduated from Liverpool University. She joined our lab in year 2009 and stayed for almost 7 years. Her main research work is on the molecular basis of insecticide resistance in Indian malaria vectors.
Congratulations Dr Cherry! We all wish you a bright future ahead of you!
06 Aug 2018
ICMR-Senior Reaearch Fellowship awarded to three PhD students
Three graduate students--Gunjan Sharma, Shobhna Mishra and Taranjeet Kaur, have received 2018-ICMR-Senior Reaearch Fellowship
Congratulations!
Bhoopal Ram
Uday Prakash
Alka Kapoor
Technical Officer
Deepa Jha, PhD
ICMR Centennary Reseach Associate
Shobhana Mishra, MSc
PhD Scholar
Taranjeet Kaur, MSc
PhD Scholar
Ankita Sindhania
PhD Scholar
Gunjan Sharma, MSc
PhD Scholar
O P Singh, PhD
Scientist G
Sneh Shalini, PhD
Technical Officer
Sonal Kale, MSc
PhD Scholar
Pre-2005
2005-09
2010-12
2013-14
2015
2016-17
2004
Singh OP, Chandra D, Nanda N, Raghavendra K, Sunil S, Sharma SK, Dua VK, Subbarao SK
Differentiation of members of the Anopheles fluviatilis species complex by an allele- specific polymerase chain reaction based on 28S ribosomal DNA sequences.
American Journal of Tropical Medicine & Hygiene, 70: 27—32, 2004
Sunil S, Raghavendra K, Singh OP, Malhotra P, Huang Y, Zheng L, Subbarao SK.
Isolation and characterization of microsatellite markers from malaria vector, Anopheles culicifacies.
Molecular Ecology Notes, 4:440—442, 2004
Singh OP, Goswami Geeta, Nanda N, Raghavendra K, Chandra D, Subbarao SK.
An allele-specific polymerase chain reaction assay for the identification of members of Anopheles culicifacies complex.
Journal of Biosciences, 29: 275—280, 2004
Sharma SK, Upadhyay AK, Haque MA, Singh OP, Adak T, Subbarao SK.
Insecticide susceptibility status of malaria vectors in some hyperendemic tribal districts of Orissa.
Current Science, 87: 1722—1726, 2004
2002
Singh OP, Raghavendra K, Nanda N, Mittal PK, Subbarao SK.
Pyrethroid resistance in Anopheles culicifacies in Surat district, Gujarat, west India
Current Science, 82:547— 550, 2002
Mittal PK, Adak T, Singh OP, Raghavendra K, Subbarao SK.
Reduced susceptibility to deltamethrin in Anopheles culicifacies sensu lato, in Ramnathapuram district, Tamil Nadu – Selection of a pyrethroid-resistant strain
Current Science, 82:185—188, 2002
Subbarao SK, Singh OP.
Biological and genetic properties of Anopheles and malaria transmission in India.
In: Trends in Malaria and Vaccine Research- the Current Scenario (Eds: D. Raghunath, R. Nayak) Tata McGraw-Hill Publishing Company Limited. New Delhi, pp. 36—43, 2002
2000
Kaur S, Singh OP, Adak T.
Susceptibility of species A, B, C of Anopheles culicifacies complex to Plasmodium yoelii yoelii and Plasmodium vinckei petteri infections.
Journal of Parasitology, 86: 1345—1348, 2000
Pathak N, Mittal PK, Singh OP, Sagar DV, Vasudevan P.
Larvicidal action of essential oils from plants against the vector mosquitoes Anopheles stephensi (Liston), Culex quinquefasciatus (Say) and Aedes aegypti (L.).
International Pest Control 42: 53—55, 2000.
Adak T, Kaur S, Singh OP.
Comparative susceptibility of different members of Anopheles culicifacies complex to Plasmodium vivax.
Transactions of the Royal Society of Tropical Medicine & Hygiene, 93: 573—577, 1999
Singh N, Singh OP, Sharma VP.
Dynamics of malaria transmission in forested and deforested regions of Mandla district, Central India (Madhya Pradesh).
2009
Singh OP, Bali P, Hemingway J, Subbarao SK, Das AP, Adak T.
PCR-based methods for the detection of L1014 kdr mutation in Anopheles culicifacies sensu lato.
Malaria Journal, 8:154, 2009
Sharma A, Parasher H, Singh OP, Adak T.
Species B of Anopheles culicifacies (Diptera: Culicidae) is reproductively less fit than Species A and C of the complex.
Acta Tropica, 112: 316-319, 2009
2008
Sohail M, Kaul A, Bali P, Raziuddin M, Singh MP, Singh OP, Dash AP, Adak T.
Alleles - 308A and -1031C in the TNF-α gene promoter do not increase the risk but associated with circulating levels of TNF-α and clinical features of vivax malaria in indian patients.
Molecular Immunology 45: 1682-1692, 2008
2007
Dash AP, Adak T, Raghavendra K, Singh OP.
The biology and control of malaria vectors in India.
Current Science 92: 1571-1578, 2007
2006
Singh OP, Chandra D, Nanda N, Htun PT, Adak T, Subbarao SK, Dash AP.
On the conspecificty of Anopheles fluviatilis S with Anopheles minimus C.
Journal of Biosciences, 31: 671-677, 2006
Goswami G, Singh OP, Nanda N, Raghavendra K, Gakhar SK, Subbarao SK.
Identification of all members of the Anopheles culicifacies complex using allele-specific polymerase chain reaction assays.
American Journal of Tropical Medicine & Hygiene 75: 454-460, 2006
Adak T, Singh OP, Nanda N, Sharma VP and Subbarao SK.
Isolation of a Plasmodium vivax refractory Anopheles culicifacies strain from India.
Tropical Medicine and International Health. 11: 197-203, 2006
2005
Adak T, Singh OP, Das MK, Wattal S, Nanda N.
Comparative susceptibility of three important malaria vectors Anopheles stephensi, Anopheles fluviatilis and Anopheles sundaicus to Plasmodium vivax.
Journal of Parasitology, 91: 79—82, 2005
Batra CP, Raghavendra K, Adak T, Singh OP, Singh SP, Mittal PK, Malhotra MS, Sharma RS, Subbarao SK.
Evaluation of bifenthrin treated mosquito nets against anopheline and culicine mosquitoes.
Indian Journal of Medical Research 121: 55-62, 2005
2012
Prajapati SK, Kumari P, Singh OP.
Molecular analysis of reticulocyte binding protein-2 gene in Plasmodium vivax isolates from India.
BMC Microbiology 12:243, 2012
Gupta P, Das A, Singh OP, Ghosh SK, Singh V.
Assessing the genetic diversity of the vir genes in Indian Plasmodium vivax population.
Acta Tropica 124: 133-139, 2012
Das A, Anvikar AR, Cator LJ, Dhiman RC, Eapen A, Mishra N, Nagpal BN, Nanda N, Raghavendra KR, Read AF, Sharma SK, Singh OP, Singh V, Sinnis P, Srivastava HC, Sullivan SA, Sutton PL, Thomas MB, Carlton J, Valecha N.
Malaria in India: The Center for the Study of Complex Malaria in India.
Acta Tropica 121: 267–273, 2012
2011
Singh OP, Dykes CL, Lather M, Agrawal OP, Adak T.
Knock-down resistance (kdr)-like mutations in the voltage gated sodium channel of a malaria vector Anopheles stephensi and PCR assays for their detection
Malar J, 10: 59, 2011
Mehrunnisa A, Adak T, Singh OP, Nanda N, Dua VK, Parasher H, Khan W.
Laboratory colonization of Anopheles fluviatilis species T and U.
J Med Entomol, 48: 395-397, 2011
Dixit J, Srivastava H, Singh OP, Saksena DN, Das A.
Multilocus nuclear DNA markers and genetic parameters in an Indian Anopheles minimus population.
Infect Genet Evol 11: 572–579, 2011
2010
Singh OP, Dykes CL, Das MK, Pradhan S, Bhatt RM, Agrawal OP, Adak T.
Presence of two alternative kdr-like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na+ channel of Anopheles culicifacies from Orissa, India.
Malar J, 9:146, 2010
Singh OP, Nanda N, Dev V, Bali P, Sohail M, Mehrunnisa A, Adak T, Dash AP.
Molecular evidence of misidentification of Anopheles minimus as Anopheles fluviatilis in Assam (India).
Acta Tropica, 113: 241–244, 2010
Surendran SN, Singh OP, Jude PJ, Ramasamy R.
Genetic evidence for malaria vectors of the Anopheles sundaicus complex in Sri Lanka with morphological characteristics attributed to Anopheles subpictus species B.
Malar J 9:343, 2010
Dixit J, Srivastava H, Sharma M, Das MK, Singh OP, Raghavendra K, Nanda N, Dash AP, Saksena DN, Das A.
Phylogenetic inference of Indian malaria vectors from multilocus DNA sequences.
Infect Genet Evol 10:755–763, 2010
Singh OP, Dykes CL, Adak T.
kdr-like mutations in the voltage gated sodium channel of a malaria vector Anopheles stephensi and development of PCR-based assays for their detection.
Malar J, 9 (Suppl 2):P67, 2010
2014
1. Kar NP, Kumar, Singh OP, Calton J, Nanda N.
A review of malaria transmission dynamics in forest ecosystems.
Parsit Vectors 7:265, 2014
2. Singh R, Singh DP, Gupta R, Savargaonkar D, Singh OP, Nanda N, Bhatt RM, Valecha N.
Comparison of three PCR-based assays for the non-invasive diagnosis of malaria: detection of Plasmodium parasites in blood and saliva.
Eur J Clin Microbiol Infect Dis. 33:1631-1639, 2014
3. Prajapati SK, Singh OP.
Identification of a vir-orthologous immune evasion gene family from primate malaria parasites.
Parasitology. 141:641-645, 2014
4. Prajapati SK, Culleton R, Singh OP.
Protein trafficking in Plasmodium falciparum-infected red cells and impact of the expansion of exported protein families.
Parasitology. 141: 1533-1543, 2014
2013
5. Mittal PK, Nanda N, Singh OP, Batra CP, Adak T.
Establishment of a focus on Anopheles fluviatilis, an important malaria vector near the National Thermal Power Corporation Project in Dadri CHC area in District Gautam Budh Nagar, Uttar Pradesh, India: a case study.
J Vector Borne Dis. 50:307-10, 2013
6. Mallick PK, Singh R, Singh OP, Singh AK, Bhasin VK, Valecha N.
Reduced heterozygosity at intragenic and flanking microsatellites of pfcrt gene establishes natural selection based molecular evolution of chloroquine-resistant Plasmodium falciparum in India.
Infect Genet Evol. 20:407-12, 2013
7. Mallick PK, Sutton PL, Singh R, Singh OP, Dash AP, Singh AK, Carlton JM, Bhasin VK.
Microsatellite analysis of chloroquine resistance associated alleles and neutral loci reveal genetic structure of Indian Plasmodium falciparum.
Infect Genet Evol. 19:164-75, 2013
8. Sharma A, Dhayal D, Singh OP, Adak T, Bhatnagar RK.
Gut microbes influence fitness and malaria transmission potential of Asian malaria vector Anopheles stephensi.
Acta Tropica, 128(1):41-7, 2013
9. Prajapati SK, Singh OP.
Remodeling of human red cells infected with Plasmodium falciparum and the impact of PHIST proteins.
Blood Cells Mol Dis. 51:195–202, 2013
10. Sunil S, Singh OP, Nanda N, Raghavendra K Reddy N. Subbarao SK.
Analysis of population genetic structure of Indian Anopheles culicifacies species A using microsatellite markers.
Parasit Vectors, 6:166, 2013
11. Mehrunissa A, Sharma A, Parasher H, Dhayal D, Singh OP, Nanda N, Adak T.
Comparative susceptibilities of species T and U of the Anopheles fluviatilis Complex to Plasmodium vinckei petteri sporogony..
J Med Entomol 50:594-597, 2013
12. Prajapati SK, Singh OP.
Insights into the invasion biology of Plasmodium vivax
Front Cell Infect Microbiol. 3:8, 2013
13. Nanda N; Singh OP, Dua VK, Pandey AC, Nagpal BN, Adak T, Dash AP, Subbarao SK..
Population cytogenetic and molecular evidence for existence of a new species in Anopheles fluviatilis complex (Diptera: Culicidae).
Infect Genet Evol 13: 218-223, 2013
2015
1. Kushwah RBS, Dykes CL, Kapoor N, Adak T, Singh OP*
Pyrethroid-resistance and presence of two knockdown resistance (kdr) mutations, F1534C and a novel mutation T1520I, in Indian Aedes aegypti.
PLoS Negl Trop Dis 9(1): e3332, 2015 [IF: 4.45]
2. Dykes CL, Kushwah RBS, Das MK, Tiwari SN, Bhatt RM, Veer V, Agrawal OP, Adak T, Singh OP*.
Knockdown resistance (kdr) mutations in Indian Anopheles culicifacies populations.
Parsit Vectors 8 8:333, 2015 [IF: 3.43]
3. Sharma D, Lather M, Mallick P, Adak T, Dang AS, Valecha N, Singh OP*
Polymorphism in drug resistance genes dihydrofolate reductase and dihydropteroate synthase in Plasmodium falciparum in some states of India.
Parasit Vectors, 8 8:471, 2015 [IF: 3.43]
4. Singh N, Mishra AK, Chand SK, Bharti PK, Singh MP, Nanda N, Singh OP, Sodagiri K, Udhyakumar V
Relative Abundance and Plasmodium Infection Rates of Malaria Vectors in and around Jabalpur, a Malaria Endemic Region in Madhya Pradesh State, Central India .
PLoS ONE 10(5): e0126932, 2015 [IF: 3.23]
5. Carlton JM*, Volkman SK, Uplekar S, Hupalo DN, Alves JMP, Cui L, Donelly M, Roos DS, Harb OS, Acosta M, Read A, Ribolla PE, Singh OP, Valecha N, Wassmer SC, Ferreira M, Escalante AA.
Population genetics, evolutionary genomics, and genome-wide studies of malaria: A view across the International Centers of Excellence for Malaria Research.
Am J Trop Med Hyg 93:87-98 [IF: 2.70]
6. Singh OP*, Dykes CL, Sharma G, Das MK.
L1014F-kdr mutation in Indian Anopheles subpictus arising from two alternative transversions in the voltage gated sodium channel and a single PIRA-PCR for their Detection
J Med Entomol 52 (1): 24-27, 2015. [IF: 1.95]
7. Lather M, Sharma D, Dong AS, Adak T, Singh OP*.
Isolation and characterization of polymorphic microsatellite markers from the malaria vector Anopheles fluviatilis species T.
J Med Entomol. 52: 408–412, 2015 [IF: 1.95]
8. Kushwah RBS, Mallick PK, Ravikumar H, Dev V, Kapoor N, Adak T, Singh OP*
Status of DDT and pyrethroid resistance in Indian Aedes albopictus and absence of knockdown resistance (kdr) mutation.
J Vector Borne Dis 52:95–98, 201, 2015 [IF: 0.81]
9. Dev V, Adak T, Singh OP, Nanda N, Baidya BK
Malaria transmission in Tripura: disease distribution & determinants.
Indian J Med Res 142:12-22, 2015 [IF: 1.39]
2016
1. Sharma D, Lather M, Dykes1 CL, Dang AS, Adak T, Singh OP*.
Disagreement in genotyping results of drug resistance alleles of the Plasmodium falciparum dihydrofolate reductase (Pfdhfr) gene by allele-specific PCR (ASPCR) assays and Sanger sequencing.
Parasitol Res 115:323-328, 2016
2. Dykes CL, Das MK, Eapen A, Batra CP, Ghsh, SK, Vijayan VA, Mishra S, Singh OP*.
Knockdown Resistance (kdr) Mutations in Indian Anopheles stephensi Populations
J Med Entomol [53: 315-20. 2016 [DOI: http://dx.doi.org/10.1093/jme/tjv242 ]
3. Rao P, Uplekar S, Kayal S, Mallick PK, Bandyopadhyay N, Kale S, Singh OP, Mohanty A, Mohanty S, Wassmer S, Carlton JM.
A method for amplicon deep sequencing of drug resistance genes in Plasmodium falciparum clinical isolates from India.
J. Clin. Microbiol. 54:1500-11. [DOI: 10.1128/JCM.00235-16]
4. Jain J, Kushwah RB, Singh SS, Sharma A, Adak T, Singh OP, Bhatnagar RK, Subbarao SK, Sunil S
Evidence for Natural Vertical Transmission of Chikungunya Viruses in Field Populations of Aedes aegypti in Delhi and Haryana States in India - A Preliminary Report.
Acta Trop. 2016 Jun 6. doi: 10.1016/j.actatropica.2016.06.004
5. Mishra N, Bharti RS, Mallick P, Singh OP, Srivastava B, Rana R, Phookan S, Gupta HP, Ringwald P, Valecha N.
Emerging polymorphisms in falciparum Kelch 13 gene in Northeastern region of India.
Malar J. 2016 Dec 3;15(1):583
2017
6. Singh R, Singh DP, Savargaonkar D, Singh OP, Bhatt RM, Valecha N.
Evidence of SYBR green I based visual loop-mediated isothermal amplification (LAMP) assay for genus and species-specific diagnosis of malaria in P. vivax and P. falciparum in endemic regions.
J Vector Borne Dis. 2017 Jan-Mar;54(1):54-60
I am a scientist (Scientist-G) working in National Institute of Malaria Research (NIMR) at Delhi.
My primary research interest focuses on identifying molecular basis of insecticide resistance in mosquitoes and drug resistance in malaria parasites, developing molecular diagnostic tools for identification of mosquitoes sibling species (which can't be differentiated morphologically) as well as for monitoring of insecticide resistance in malaria vectors.
Ongoing Projects:
Molecular basis of insecticide resistance in Aedes aegypti—DBT funded project (2014-2018)
Pyrethroid resistance in Indian malaria vectors Anopheles stephensi and Anopheles culicifacies —DST-SERB funded project (2018-2020)
Publications
About
Molecular Biology Division
Laboratories # 209, 210 and 317
Office: Room # 323
National Institute of Malaria Research
Sector 8, Dwarka
New Delhi-110077
India
Email: Singh(*)mrcindia.org
Tel: +91-11-25307323
Mobile: +91-98 68 00 33 55
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